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  1. Resuspend lyophilized EPS in minimum amount of buffer (10mM Tris pH 7, 5mM MgCl2)
  2. Add DNAse and RNAse to 0.1 mg/mL. Incubate 37oC, 5 hrs.
  3. Add Proteinase K to 0.1 mg/mL.  Incubate 37oC O/N.
  4. Phenol extract 2X (Phenol with 10mM Tris, pH 6.7 (I use pH 8.0), 1mM EDTA), followed by 1 chloroform extraction.
  5. Precipitate EPS with 3 volumes of ethanol, centrifuge, wash as before.
  6. Resuspend pellet in minimum amount of H2O, lyophilize.