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- Resuspend lyophilized EPS in minimum amount of buffer (10mM Tris pH 7, 5mM MgCl2)
- Add DNAse and RNAse to 0.1 mg/mL. Incubate 37oC, 5 hrs.
- Add Proteinase K to 0.1 mg/mL. Incubate 37oC O/N.
- Phenol extract 2X (Phenol with 10mM Tris, pH 6.7 (I use pH 8.0), 1mM EDTA), followed by 1 chloroform extraction.
- Precipitate EPS with 3 volumes of ethanol, centrifuge, wash as before.
- Resuspend pellet in minimum amount of H2O, lyophilize.