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Label base of LB plate with culture name and dilutions (see sample in Figure 1).
Extract 10 mL of culture with highest dilution (i.e. lowest cell concentration) from microtitre plate and pipette onto LB plate, in area labeled with corresponding dilution. Extract another 10 mL of culture of same dilution and pipette onto LB plate, next to the first 10 m Make sure that the two spots do not touch each other. (Depending on the experiment at hand, you may wish to do more than 2 replicate spots.)
Repeat step 11, transferring culture from microtitre plate to LB plate, in order of decreasing culture dilution (i.e. increasing cell concentration). By proceeding from highest dilution to lowest dilution, the same pipette tip may be used throughout the plating process.
After plating is complete, leave plates on bench top to air dry.
Invert plates and incubate overnight.