Prices shown below are estimates based on typical pricing. According to UT System and UT Austin policy, the GSAF bills for actual expenses can vary with the size and scope of each project. The GSAF does not guarantee read counts but we will try our best to meet customer request when we prepare the libraries, however we will not make up any read counts for customer prepared libraries.
- DNA and RNA Library Preps
- Single Cell
- Metagenomics (ribosomal gene sequencing)
- Quality Control by BioAnalyzer
- Tag-Seq Services
- Visium and Xenium
Single Cell, scRNA-Seq Seq
- Currently offering 10X Genomics of Parse Biosciences
- Sequencing will be offered on the NextSeq or NovaSeq
- Sequencing charges are independent of library prep charges
| 10X Library Preparation | Cost per sample Internal | Cost per sample External |
|---|---|---|
| 1 Per Sample, Library Prep Only, 3' or 5' GEM-X (20k cells) | $2120 | $2685 |
| 1 Per sample, 10x FLEX RNA profiling (fixed, 20k cells) | $1465 | $1853 |
| Add Feature Barcode (must use compatible 10X partners) | $274 | $346 |
| Add TCR or BCR Amplification | $254 | $312 |
| 1 Chip per 4 samples | $301 | $382 |
| Parse Lib Prep | Cost per project Internal | Cost per sample External |
|---|---|---|
| Parse WT Mini (10K cells) | $7,055 | $8,932 |
| Parse WT (100K cells) | $13,450 | $17,039 |
| Parse WT Mega (1M cells) | $22,712 | $28,784 |
Please inquire if you are interested in adding TCR/BCR to a Parse single cell library
Tag-Seq Services
- TagSeq is a service offered by the GSAF, this is a 3' RNA based library prep utilizing the intrinsic properties of a reverse transcriptase
- RNA submitted must be high quality and must have the concentrations normalized, preferred concentration for the RNA is between 10-100 ng/ul providing at least 25 microliters
- Samples must be submitted in 96 well plates
- If Trizol/Tri-Reagent was used for extraction samples should go through a column clean up
- IMPORTANT NOTES!
- The GSAF optimized the TAG-Seq service on human RNA, other organisms may require different conditions however we do not customize this prep
- This service was designed to offer researchers the opportunity to run large scale RNA-Seq projects at an affordable price, however if you are not familiar with this service I advise against it, the GSAF is not responsible for failed projects if all internal controls perform well.
- If you do not follow all of our recommendations for this service your data will be impacted
- If you are not familiar with bioinformatics I highly urge you to use our Bioinformatic team
Samples should be plated starting at A1 then plate the samples vertically, A1 to H1, then A2 to H2 and so on.....Do not skip wells, you will be charged for wells that are skipped and we will not process samples that are plated A1 to A12.
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**IF min sample number is not met $240 added for internal and $303 for external (20 Sample Minimum)
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DNA and RNA library preps
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DNA ~400 bp insert size. Price includes final QC by BioAnalyzer and qPCR. See this web page for sample input guidelines.
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| Type of Library Preparation Procedure | Internal / UT Network | External Academic |
| Standard DNA library | $101 | $127 |
| RNA directional WITH poly-A enrichment | $206 | $260 |
| RNA directional WITH ribosomal removal | $301 | $381 |
| Small RNA or RNA with no poly-A or ribosomal removal | $141 | $179 |
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16S and ITS Metagenomics, ONLY for INTERNAL UT Austin Researchers.
Please note, these prices are for library prep only.
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Prices are listed per sample to include library preparation from genomic DNA and sequencing. For jobs with less than 96 samples, a flat fee per project is charged as shown.
Prices vary slightly depending on the actual number of samples processed, so the prices are provided for reference and planning purposes. Submit a job request to get accurate, approved pricing.
| Number of Samples | Internal / UT Network | External Academic |
|---|---|---|
| 96 or more | $35 | $45 |
| 1-95 samples | Add $369 / project | Add $464 / project |
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Note: if you are submitting a mixed population (e.g. bacterial & fungal, or bacterial & eukaryote host, etc.) you should assay your samples before submission to ensure you have the proper concentration of the target DNA, not simply total DNA. Samples submitted with insufficient target DNA may result in excessive primer-dimer or off-target products which produce no useful data. These will still be charged according to the pricing table.
Spatial Transcriptomics
The GSAF offers both whole transcriptome and targeted spatial transcriptomics. Please consult with the lab to discuss these options.
- 10X Chromium iX
- 10x CytAssist (HD assays)
- 10x Xenium Platform
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