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Sequence | TruSeq name | NEXTFlex number |
|---|---|---|
ATCACG | TSBC01 | NFBC07 |
CGATGT | TSBC02 | NFBC01 |
TTAGGC | TSBC03 | NFBC08 |
TGACCA | TSBC04 | NFBC02 |
ACAGTG | TSBC05 | NFBC03 |
GCCAAT | TSBC06 | NFBC04 |
CAGATC | TSBC07 | NFBC05 |
ACTTGA | TSBC08 | NFBC09 |
GATCAG | TSBC09 | NFBC10 |
TAGCTT | TSBC10 | NFBC11 |
GGCTAC | TSBC11 | NFBC12 |
CTTGTA | TSBC12 | NFBC06 |
AGTCAA | TSBC13 | NFBC13 |
AGTTCC | TSBC14 | NFBC14 |
ATGTCA | TSBC15 | NFBC15 |
CCGTCC | TSBC16 | NFBC16 |
GTAGAG | TSBC17 | NFBC17 |
GTCCGC | TSBC18 | NFBC18 |
GTGAAA | TSBC19 | NFBC19 |
GTGGCC | TSBC20 | NFBC20 |
GTTTCG | TSBC21 | NFBC21 |
CGTACG | TSBC22 | NFBC22 |
GAGTGG | TSBC23 | NFBC23 |
GGTAGC | TSBC24 | NFBC24 |
ACTGAT | TSBC25 | NFBC25 |
ATGAGC | TSBC26 | NFBC26 |
ATTCCT | TSBC27 | NFBC27 |
CAAAAG | TSBC28 | NFBC28 |
CAACTA | TSBC29 | NFBC29 |
CACCGG | TSBC30 | NFBC30 |
CACGAT | TSBC31 | NFBC31 |
CACTCA | TSBC32 | NFBC32 |
CAGGCG | TSBC33 | NFBC33 |
CATGGC | TSBC34 | NFBC34 |
CATTTT | TSBC35 | NFBC35 |
CCAACA | TSBC36 | NFBC36 |
CGGAAT | TSBC37 | NFBC37 |
CTAGCT | TSBC38 | NFBC38 |
CTATAC | TSBC39 | NFBC39 |
CTCAGA | TSBC40 | NFBC40 |
GACGAC | TSBC41 | N/A |
GCGCTA | N/A | NFBC41 |
TAATCG | TSBC42 | NFBC42 |
TACAGC | TSBC43 | NFBC43 |
TATAAT | TSBC44 | NFBC44 |
TCATTC | TSBC45 | NFBC45 |
TCCCGA | TSBC46 | NFBC46 |
TCGAAG | TSBC47 | NFBC47 |
TCGGCA | TSBC48 | NFBC48 |
NOTE that TSBC41 is hamming distance 2 away from both TSBC31 and TSBC11; all others are hamming distance >=3.
Some additional 5 bp barcodes can be found here:http://comailab.genomecenter.ucdavis.edu/index.php/Barcodes
An additional set of 6 bp barcodes that have hamming distance of at least 3 from the table above of 49 barcodes is as follows (NOTE: these have NOT been tested on the sequencer):
Sequence | GSAF name |
|
|---|---|---|
AAACAC | UTBC50 |
|
TGAAGG | UTBC51 |
|
AACATA | UTBC52 |
|
CGCGTC | UTBC53 |
|
GATACA | UTBC54 |
|
GGTGTG | UTBC55 |
|
TAAGAA | UTBC56 |
|
AGCGAG | UTBC57 |
|
CGGTTA | UTBC58 |
|
AGCTTT | UTBC59 |
|
TGGTCT | UTBC60 |
|
TATCCC | UTBC61 |
|
TGTCGT | UTBC62 |
|
CCCCAC | UTBC63 |
|
ATACGA | UTBC64 |
|
CCCTTG | UTBC65 |
|
ACCGGC | UTBC66 |
|
TTACTG | UTBC67 |
|
GGAACT | UTBC68 |
|
GTTATT | UTBC69 |
|
AAAAGT | UTBC70 |
|
AAGGGA | UTBC71 |
|
AAGTAT | UTBC72 |
|
ACATCT | UTBC73 |
|
ACGATT | UTBC74 |
|
ACGCCG | UTBC75 |
|
ACTCTC | UTBC76 |
|
AGAATC | UTBC77 |
|
ATTGGG | UTBC78 |
|
CCGCGT | UTBC79 |
|
CGCCCT | UTBC80 |
|
CTGCAG | UTBC81 |
|
GAAGTT | UTBC82 |
|
GCACCC | UTBC83 |
|
GCAGGA | UTBC84 |
|
GCCGCG | UTBC85 |
|
GGCGGT | UTBC86 |
|
GTATTA | UTBC87 |
|
TACGTG | UTBC88 |
|
TCACAT | UTBC89 |
|
TCTATA | UTBC90 |
|
TGCAAA | UTBC91 |
|
TGGCAC | UTBC92 |
|
TGTTAG | UTBC93 |
|
TTCTAT | UTBC94 |
|
Excruciating details - USE WITH CAUTION - RNA PCR primers are NOT current as of Dec. 2011
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