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Table of Contents

Your Instructors

  • Anna Battenhouse, Associate Research Scientist, abattenhouse@utexas.edu, Biomedical Research Computing Facility Manager, and Marcotte lab staff

    • BA English literature, 1978

    • Commercial software development 1982 – 2007

    • Joined Iyer Lab 2007 (“retirement career”)

    • BS Biochemistry, UT Austin, 2013

    • Joined the Biomedical Research Computing Facility (BRCF) and Marcotte Lab summer 2017
    • BS Materials Science & Engineering, UC Berkeley, 2017
    • Student Researcher in France and Portugal 2017 - 2018
    • Research Assistant in single cell genomics, UT Southwestern 2019-2021
    • 4rd year graduate student in the Marcotte Lab 
    • Research Interests: biomaterials, developmental biology, and bioinformatics
    • Also affiliated with
      • Bioinformatics Consulting Group (BCG)
      • Genome Sequencing and Analysis Facility (GSAF)
      • Cryo Electron Microscopy core facility (CryoEM)

    Daryl Barthdaryl.barth@utexas.edu

  • Matt Bramble, matthew.bramble@austin.utexas.edu, Associate Research Scientist
    • Master’s degrees from UT Austin in Molecular Biology and Statistics
    • 10 years of experience with R and Python
    • Recently joined the CBRS Bioinformatics Consulting Group after six years at MD Anderson Cancer Center analyzing a wide range of NGS data in epigenomics
    • Areas of expertise include: Hi-C (chromatin conformation) analysis, mouse somatic variant analysis, and single cell RNAseq analysis

About the Iyer Lab (where Anna learned NGS)

http://iyerlab.org/

Dr. Vishy Iyer, PI

Main focus is functional genomics

    • large-scale transcriptional reprogramming
      in response to diverse stimuli
    • Encode consortium collaborator
    • works in human and yeast


Research methods include
  • microarrays (Dr. Iyer was co-inventor)

  • high-throughput sequencing (since 2007)
    • especially ChIP-seq, RNA-seq
    • also miRNA-seq, RIP-seq, MNase-seq ...
    • have ~2>2,000 NGS datasets

Communication

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For online attendees, you can also post your question to the Zoom chat. We'll sometimes use breakout rooms when troubleshooting problems you run into, if so, TA Daryl Barth Matt Bramble will assign you to one.

Getting help

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Large and growing datasets

NGS methods produce staggering amounts of data!

Typical dataset these days

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