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While checking the number of reads a file has can solve some of the most basic problems, it doesn't really provide any direct evidence as to the quality of the sequencing data. To get this type of information before starting meaningful analysis other programs must be used.
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| Place your sticky note on your computer when you have made it this far and start looking over the fastqc links below. Once everyone has caught up we will go over this together. |
FASTQ Evaluation Tools
The first order of business after receiving sequencing data should be to check your data quality. This often-overlooked step helps guide the manner in which you process the data, and can prevent many headaches that could require you to redo an entire analysis after they rear their ugly heads.
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