...
- Identify Highly Variable Features
Scale the Data
- Select Dimensions using PCA
- Cluster the cells
- Find markers for each cluster
- Visualize the clusters using tSNE or UMAP
You can get the data and R script from ls5 here:
/work/01184/daras/rnaseq_course_2016/day_5_single_cell_data
The script: seuratTotalScript5k.R
The data: filtered_feature_bc_matrix