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  5. SSC Intro to NGS Bioinformatics Course

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Dhivya Arasappan

Scott Patrick Hunicke-Smith

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  • General introduction (SPHS)
    • Introduction to Bioinformatics Prezi
  • Getting started at TACC
  • Linux refresher (SPHS)
  • Using TACC's Lonestar Cluster (SPHS)
    • Diagram of Lonestar's directories
    • Diagram of running a job on Lonestar
  • Introduction to next-gen sequencing technologies (SPHS)
    • Official Illumina video | Another Illumina video
    • Broad Center GA Boot Camp
    • GSAF adaptor and barcode sequence resource
  • Application: Example genome variant calling pipeline (JB)
  • Evaluating your raw sequencing data (JB/AB)
  • Introduction to Mapping and Tutorial
    • (JB)
    • Mapping tutorial (bowtie2, BWA) (JB/GC)
  • Integrative Genomics Viewer (IGV) tutorial (Part 1) (JB)
  • start diploid mapping (copy from Day 2 content)

...

  • Introduction to genome variation
  • (JB)
  • Variant calling tutorial (SAMtools) (JB)
  • Integrative Genomics Viewer (IGV) tutorial (Part 2) (JB)
  • Visualize mapped data at UCSC genome browser (AB)
  • (JB)
  • Calling variants in diploid genomes (SPHS)
  • Variant calling with GATK (SPHS)
  • Annotating variants (SPHS)

...

General
Content
Integrations
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