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Latest version on Fourierseq: 0.1.18 installed 9/26/11 by SPHS

Useful samtools utilies:

1. samtools idxstats : This tool will provide statistics about how many reads have aligned to each sequence/chromosome in the reference genome. The input bam file must be sorted and indexed.

Code Block

samtools idxstats <in.bam>

2. samtools flagstat : Simple stats about how many reads mapped to the reference, how many reads were paired properly etc. The input bam file must be sorted and indexed.

Code Block

samtools flagstat <in.bam>

Example:

1. samtools mpileup -Euf reference.fna aln1.bam aln2.bam | bcftools view -bvcg - > var.raw.bcf

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