...
- In an actual RNAseq analysis, you might want to trim stray adaptor sequences from your data using a tool like the FASTX-Toolkit, FAR, or cutadapt before aligningthe tools discussed in Evaluating your raw sequencing data.
- You can get a lot more information from RNAseq data than you could from a microarray experiment. You can map transcriptional start sites, areas of unexpected transcription, splice sites, etc. - all because you have full sequence information that we have barely used in this example.
- You can call variants from mapped RNAseq data, just be aware that many regions will have no coverage (because they are not expressed as RNA).
...