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The Integrative Genomics Viewer (IGV) from the Broad Center allows you to view several types of data files involved in any NGS analysis that employs a reference genome, including how reads from a dataset are mapped, gene annotations, and predicted genetic variants.

 

Get your data

We are going to look at two mapping results- for samples C1_R1 and C2_R2.  Download the corresponding bam files and index files from here onto your computer.

Some useful IGV Features

  1. SNPS
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  2. Coverage tracks- to see coverage in one sample vs another acorss a certain location.
  3. All sorts of views for paired end data.  These help us find analogous pair mappings, split mappings etc.
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Launching IGV

Warning

For the remainder of the tutorial, work on your local machine. NOT TACC!

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