...
- Tools Used:
- Trans-Proteome Pipeline: (Deutsch E. W. et. al. )
- XML output of all peptide and protein hits.
- Trans-Proteome Pipeline: (Deutsch E. W. et. al. )
2. Peptide hits validation
Validate the peptide hits returned by the mass-spectrometry peptide identification algorithms
- Deliverables:
- reports generated by peptideProphet
- Tools Used:
- peptideProphet: (Nesvhizskii et. al. ) used to validate peptide hits:
- pepXML output file that has list of validated peptide hits.
- pepXML output file that has list of validated peptide hits.
- peptideProphet: (Nesvhizskii et. al. ) used to validate peptide hits:
3. Protein hits validation
Validate the protein hits using the pepXML peptideProphet output
- Deliverables:
- reports generated by iProphet
- Tools Used:
- iProphet: (Nesvhizskii et. al. ) used to identify high-confident protein hits
- protXML output file that has list of validated protein hits.
- iProphet: (Nesvhizskii et. al. ) used to identify high-confident protein hits