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  • Jump to the next point of interest. Click on a track name on the left side of the window (Ex: bowtie.vcf), then it will be selected. You can then use control-f and control-b to jump forward and backward within that list of features. Try this on the variant calls.
  • Jump right to a gene. Typing its name in to the search box. Try "topA".
  • Navigate more quickly. The home and end keys jump a page backward and forward.
  • Load multiple VCF and BAM alignments at once. Try this to compare a few different regions between the bowtie and BWA results.
  • Change the appearance of genes. Right click on the gene track and try "expanded". Experiment with the other options.
  • Change the appearance of reads. Right click on a BAM track and choose "show all bases" and "expanded". Experiment with the other options.

Exercises

  • Check out the rbsA gene region? What's going on here?
  • What is going on in the pykF gene region? You might see red read pairs. What does that mean?
  • Go to coordinate 2,698,092. Compare the bowtie and BWA alignments. Can you explain what's going on here?
  • Go to coordinate 475,263. What's the story here?