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  5. SSC Intro to NGS Bioinformatics Course

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Jeffrey E Barrick

Jeffrey E Barrick

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  • Mapped read data evaluation (SAMtools)
  • Installing and compiling tools on Unix
  • Integrated pipeline for microbial genome re-sequencing analysis (breseq)
  • Calling variants in multiploid genomes and mixed populations (FreeBayes)
  • Practical advice about some things to watch out for with short read re-sequencing data
  • Comparing variants across samples (VCFtools)

Day 3: RNA-seq

  • simple quantitationDifferential gene expression analysis

Day 4: Assembly

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