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  1. Content
  2. Bioinformatics Team Home
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  4. Training
  5. SSC Intro to NGS Bioinformatics Course

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Scott Patrick Hunicke-Smith

Jeffrey E Barrick

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Aug 19, 2012

Aug 19, 2012

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  • Installing Linux virtual machine on Windows (DP)
  • Sharing Linux tricks (SPHS)
  • Making it easier to work on TACC from a Mac (JB+)

Extras

  • Running Unix & Perl for Biologists tutorial at TACC

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  • Introduction to genome variation
  • (JB)
  • Variant calling tutorial (SAMtools) (JB)
  • Integrative Genomics Viewer (IGV) tutorial (Part 2) (JB)
  • Image Added (JB)
  • Visualize mapped data at UCSC genome browser (AB)
  • Image Removed (JB)
  • Calling variants in diploid genomes (SPHS)
  • Variant calling with GATK (SPHS)
  • Annotating variants (SPHS)

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  • Mapped read data evaluation (SAMtools) (SPHS)
  • Genome variation in mixed samples (FreeBayes, deepSNV) (JB)
  • Identifying structural variants (SVDetect)
  • Practical advice - short read re-sequencing data (JB)
  • SRA toolkit, more UCSC Genome Browser (AB)

Day 3: RNA-seq

  • Intro materials - (SPHS - JB/DA)
  • Differential gene expression analysis (JB - bedtools & R)
  • Differential expression with splice variant analysis
  • MvA, volcano, heat maps, maybe goseq (R), cumberbund - check R version (SPHS/DA)

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  • Identifying mutations in microbial genomes (breseq) (JB)

Enrichment module (4:30-5:30)

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General
Content
Integrations
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