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From the main window of IGV, click on File ? Import GenomeGenomes ? Create .genome File... and you should be presented with the following window.

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You can do this from within IGV:

  1. Choose File Tools ? Run igvtools....
  2. Choose "index" from the commands drop-down menu.
  3. Select your *.vcf file (Ex: bowtie.vcf) for "Input File"
  4. Click the "run" button.

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