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Table of Contents

Communication

Post its

Green post-it – I'm good at the moment.

Pink post-it – I need a bit of help.

Conventions

If you see a block of text like this:

Code Block
languagebash
titleExample code block
ls -h

it means, type the command ls -h into a terminal window, hit return, and see what happens.

We intend this course to offer as much self-learning as possible. Consequently, you'll find many sections like this - click on the triangle to expand them:

Expand
Hint
Hint

Hint sections will provide you some guidance on what to do next, but will not spell it out.

and some sections like this:

Expand
Solution
Solution

Solution sections will contain the commands so that you could copy-and-paste them if you have to. They will represent one method of answering the question – but there are often many ways to skin a cat!

Your Instructors

  • Anna Battenhouse, Associate Research Scientist, abattenhouse@utexas.edu

    • BA English literature, 1978

    • Commercial software development 1982 – 2005

    • Joined Iyer Lab 2007 (“retirement career”)

    • BS Biochemistry, UT Austin, 2013

    • Joined Marcotte lab and Biomedical Research Support Facility (BRCF) in summer 2017
  • Haridha Shivram, haridh@utexas.edu

    • 6th year graduate student in the Iyer Lab

  • Claire McWhite, claire.mcwhite@utexas.edu

...

http://iyerlab.org/

Dr. Vishy Iyer, PI

Main focus is functional genomics

    • large-scale transciptional reprogramming
      in response to diverse stimuli
    • Encode consortium collaborator
    • work in human and yeast


Research methods include
  • microarrays (Dr. Iyer was co-inventor)

  • high-throughput sequencing (since 2007)
    • especially ChIP-seq
    • also RNA-seq, RIP-seq, MNase-seq ...
    • we now have nearly 2,000 NGS datasets

Communication

Post its

Green post-it – I'm good at the moment.

Pink post-it – I need a bit of help.

Conventions

If you see a block of text like this:

Code Block
languagebash
titleExample code block
ls -h

it means, type the command ls -h into a terminal window, hit return, and see what happens.

We intend this course to offer as much self-learning as possible. Consequently, you'll find many sections like this - click on the triangle to expand them:

...

Hint sections will provide you some guidance on what to do next, but will not spell it out.

and some sections like this:

...

Course goals

  • Hands-on, tutorial style – learn by doing
    • common bioinformatics tools & file formats
  • Introduce NGS vocabulary
    • both high-level view and practice with specific tools
  • Cover the NGS basics
    • the first few things you'll do after receiving raw sequences
      • raw sequence preparation
      • alignment to reference
      • basic alignment analysis
  • Understand and practice required skills
    • Get you comfortable with Linux and TACC – your best "frenemies"
    • Make you self-sufficient enough in 4 days to become experts over time
    • Show some "best practices" for working with NGS data

...