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Table of Contents

Your Instructors

We Most of us are members (or alumni) of the functional genomics lab of Vishwanath Iyer, UT Austin.

  • Anna Battenhouse, Associate Research Scientist, Iyer Lab, abattenhouse@utexas.eduDr. Daechan Park, Post-doctoral fellow, Georgiou Lab, daechan.park@utexas.edu
    • BA English literature, 1978
    • Commercial software development 1982 – 2005
    • Joined Iyer Lab 2007 (“retirement career”)
    • BS Biochemistry, 2013
  • Amelia Weber Hall, Graduate Student, Iyer Lab, ameliahall@utexas.edu
    • 5th year Microbiology graduate student
    • Laboratory Technician at UT 2007-2010
    • BS Molecular Genetics, 2007
  • Nathan Abell, Research Assistant, Iyer Xhemalce Lab, abell.nathan@gmail.comAmelia Weber Hall
    • Undergraduate researcher in Iyer Lab 2011-2013
    • BS Molecular Biology, UT, 2013
    • Research Assistant
  • Dakota Derryberry, Graduate Student, Wilke Lab, dakotaz@utexas.edu
    • ???

About the Iyer Lab

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http://iyerlab.org/

  • Main focus is functional genomics
    • large-scale transciptional reprogramming in response to diverse stimuli
    • Encode consortium collaborator
    • work in human and yeast
  • Research methods include
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    • high-throughput sequencing (since 2007)
      • especially ChIP-seq
      • also RNA-seq, RIP-seq, MNase-seq ...
      • we now have > 1,500 NGS datasets
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Goals

  • Hands-on, tutorial style – learn by doing
  • Cover the NGS tool basics – the first few things you'll do after receiving raw sequences
  • Get you comfortable with Linux and TACC – your best "frenemies"
  • Make you self sufficient in 4 days to become experts over time
  • Show some "best practices" for working with NGS data

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Pink post-it – I need a bit of help.

Etherpad

Link to Etherpad: https://etherpad.mozilla.org/yUFlDtWv2c

Use this to post any questions you have about the lessons and tutorials.

Conventions

Text that you find in courier font refers to a program or file name on a computer.

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Solution
Solution

Solution sections will contain the commands so that you could copy-and-paste them if you have to. They should be exactly accurate.

About the Iyer Lab

http://iyerlab.org/

  • Main focus is functional genomics
    • large-scale transciptional reprogramming in response to diverse stimuli
    • Encode consortium collaborator
    • work in human and yeast
  • Research methods include

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  • Anna's background
    • BA English literature, 1978
    • Commercial software development 1982 – 2005
    • Joined Iyer Lab 2007 (“retirement career”)
    • BS Biochemistry, 2013
  • Daechan's background
    • BS Biology, Seoul National University, 2008
    • Ph.D Cell and Molecular Biology, UT Austin, 2014
    • Baby sitting, Current

 

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