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Can you figure out how to trim only the first 16 bases off the reads using fastp?
Next step:
You should now have 2 new .fastq files which we will use to call variants in: DED110_SSCS.fastq, and DED110_all.trimmed.fastq. You should take these files into a more in depth breseq tutorial for comparisons of the specific mutations that are eliminated using the error correction (SSCS). Link to other tutorial.
Return to GVA2022GVA2023 course page.