...
3. The Sequence Duplication Levels report, which helps you evaluate library enrichment / complexity. But note that different experiment types are expected to have vastly different duplication profiles.
DETOUR: What are PCR duplicates?
- The Overrepresented Sequences report, which helps look for dominant sequences.
5. Adapter content report, which tells you about adapter contamination.
...
| Expand | ||
|---|---|---|
| ||
The Per base sequence quality report shows that trimming is not necessarythe last 10 bases may be a good idea. |
Let's look at tools to do such manipulations to fastqc files, if we have to.