Minimal Salts Vitamin Pyruvate Medium
| Chemical | g/L |
|---|---|
| (NH4)2SO4 | 1 |
| MgSO4*7H2O | 0.06 |
| CaCl2*2H2O | 0.06 |
| KH2PO4 | 0.02 |
| Na2HPO4 | 0.03 |
| HEPES | 2.383 |
| 10 mM FeSO4 | 1.0 mL |
- Adjust pH to 6.0 with NaOH.
- For solid medium, add 16 g agar per liter.
- Autoclave.
- For the 1X 1-L solution, cool to ~50ºC in a water bath and aseptically add the following filter-sterilized solutions:
...
20% sodium pyruvate, 30 mL OR 40% sodium pyruvate, 15 mL
| Chemical | mg/L | mg/500 mL |
|---|---|---|
| Biotin | 20 | 10 |
| Folic Acid | 20 | 10 |
| Thiamine HCl | 50 | 25 |
| D-(+)-Calcium pantothenate | 50 | 25 |
| Vitamin B12 | 1 | 0.5 |
| Riboflavin | 50 | 25 |
| Nicotinic acid | 50 | 25 |
| Pyridoxine HCl | 100 | 50 |
| p-Aminobenzoic acid | 50 | 25 |