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- Always wear gloves
- Scrub dishes thoroughly with a brush, Alconox, and hot water. Make sure that all precipitate or stains are removed from the glassware. Stubborn stains can be soaked overnight or put in the microwave for a few seconds.
- Rinse 5 times with tap water. Rinse 2 times with deionized water.
- It is very important that all traces of soap be removed from glassware. Therefore, do not skimp on the rinsing steps!
- Let dishes air dry on drying rack.
- Test tubes can be dried upside down in a test tube rack. The Morten closures (plastic or metal caps) can be air-dried in a wire basket with a paper towel underneath it.
- When the dishes are clean and dry, they can be put into cabinets. Cabinets are labeled with the type of glassware that should be stored in them.
Acid Bathing
- When glassware was used with metals that are hard to remove, they can be soaked in an acid bath
- Be sure not to leave glassware in the acid bath for longer than 24 hours, as this can damage the glassware
- Rinse the glassware as best you can
- Wearing acid gloves, slowly submerge your glassware such that none of the liquid in the bath splashes up or bubbles excessively
- Allow your glassware to soak no longer than 24 hours
- Wearing acid gloves, slowly remove your glassware from the acid bath
- Rinse 5 times with tap water and 2 times with deionized water
- Let glassware air dry on drying rack
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- Put the pipet washer in the sink and attach the hose to the inlet.
- Place an Alconox tablet into the water and turn the water on at a low flowrate.
- Make sure that the pipets are placed into the basket with the tip (narrow end) end up. Add the pipets to the washer.
- Allow the washer to cycle 12-15 times, or until the pipets are thoroughly free of detergent.
- Cleaned pipets can be placed into a clean receptacle until they can be dried and autoclaved.
Putting Dishes Away
- When the dishes are clean and dry, they can be put into cabinets. Cabinets are labeled with the type of glassware that should be stored in them.
Aseptic Technique
- Dark-striped autoclave tape on a container indicates that whatever is inside is sterile and should remain as such
- When extracting something from a sterile container, minimize the amount of time that its lid is off, use only sterile instruments to extract liquids, and shake out solids
Biosafety Hood
- Turn on the vent on the hood and allow it to run for at least 10 minutes before use
- Spray your gloved hands with alcohol and rub it in
- Spray and wipe the inside surface of the hood with alcohol
Packing Pipet Tips
- Wear gloves.
- Pack tips into the boxes.
- Put autoclave tape on each box.
- Put into autoclavable container and autoclave on a gravity cycle (20 minutes steam + 10 minutes drying time).
- Place autoclaved boxes on the shelf when dry.
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