There is some publicly available data available containing single cell RNA-Seq data for 5k immune cells as well as a script that runs the Seurat workflow to define cell-type clusters in this data.

The script does the following:

• Load Data and Create Seurat Object
• Normalize Data Using SCTransform and Regress out Genes Related to Cell Cycle

• Identify Highly Variable Features

• Scale the Data

• Select Dimensions using PCA
• Cluster the cells
• Find markers for each cluster
• Visualize the clusters using tSNE or UMAP