Mouse Colonies 2021
Colony Long Name | Strain | Date Ordered | Source | Mouse Description | Reference | Breeding Scheme | Genotyping Strain (Transnetyx) | EAOK Notes | Date Terminated |
|---|---|---|---|---|---|---|---|---|---|
Gabba Rho1 |
| 5/4/2012 | JAX 010535 | Gaba rho1 KO. Targeted deletion of exon 1 (and surrounding sequence) of GABA rho1 gene. Neo cassette remains. | Zheng W; Xie W; Zhang J; Strong JA; Wang L; Yu L; Xu M; Lu L. 2003. Function of gamma-aminobutyric acid receptor/channel rho 1 subunits in spinal cord. J Biol Chem 278(48):48321-9. | YES - to check its is actually just +/+ |
|
| 8/1/2013 |
MYD88-FLOXED | B6.129P2(SJL)-Myd88^tm1Defe/J | 10/5/2011 | JAX 008888 | Conditional knockout. Exon 3 of Myd88 gene is flanked by loxp sites. | Hou B; Reizis B; Defranco AL. 2008. Toll-like Receptors Activate Innate and Adaptive Immunity by using Dendritic Cell-Intrinsic and -Extrinsic Mechanisms. Immunity 29(2):272-82. |
|
|
| 2/10/2016 |
Scn4b flox |
| 1/24/2014 | Homanics | Scn4b flox. Exon 2 of Scn4b gene is flanked by loxp sites. No neo cassette remaining. |
|
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|
| 5/14/2016 |
CD.14 | B6.129S-Cd14<tm1Frm>/J | 6/15/2007 | JAX 003726 | CD14 KO. A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt the entire Cd14 coding region, 4.0 kb of upstream sequence and 2.5 kb of downstream sequence. | Moore KJ; Andersson LP; Ingalls RR; Monks BG; Li R; Arnaout MA; Golenbock DT; Freeman MW. 2000. Divergent response to LPS and bacteria in CD14-deficient murine macrophages. J Immunol 165(8):4272-80. | KO breeding |
|
| 11/14/2016 |
Tlr2 | B6.129-Tlr2^tm1Kir/J | 10/5/2011 | JAX 004650 | Tlr2 KO. A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt sequence of the targeted gene encoding the C-terminus of the extracellular and part of the transmembrane domains. | Wooten RM; Ma Y; Yoder RA; Brown JP; Weis JH; Zachary JF; Kirschning CJ; Weis JJ. 2002. Toll-like receptor 2 is required for innate, but not acquired, host defense to Borrelia burgdorferi. J Immunol 168(1):348-55. | Homozygous breeding |
|
| 11/14/2016 |
B6.B10.TLR4 | B6.B10scn-TLR4lps/JthJ | 1/16/2013 | JAX 007227 | The Tlr4Lps-del spontaneous mutation corresponds to a 74723 bp deletion that completely removes the Tlr4 coding sequence. No mRNA or protein is expressed. | Poltorak A; He X; Smirnova I; Liu MY; Huffel CV; Du X; Birdwell D; Alejos E; Silva M; Galanos C; Freudenberg M; Ricciardi-Castagnoli P; Layton B; Beutler B. 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 282(5396):2085-8. PubMed: 9851930MGI: J:51522 Vogel SN; Hansen CT; Rosenstreich DL. 1979. Characterization of a congenitally LPS-resistant, athymic mouse strain. J Immunol 122(2):619-22. PubMed: 368244MGI: J:6086 | Homozygous Breeding |
|
| 11/14/2016 |
TRPM5 KO | ? | ? | ? |
|
| Homozygous breeding |
|
| 1/30/2017 |
IKK Beta | Ikkbeta F/F | 6/22/2011 | University of California San Diego | Conditional knockout. Exon 3 of IKKB gene is flanked by loxp sites. | Zhi-Wei Li, Sidne A. Omori, Tord Labuda, Michael Karin and Robert C. Rickert; IKKb Is Required for Peripheral B Cell Survival and Proliferation. J Immunol 2003; 170:4630-4637 | Homozygous breeding |
|
| 2/7/2017 |
LYZ2-CRE-IKKB-N3 |
| 12/2/2013 | MENDY | Conditional knockout. Exon 3 of IKKB gene is flanked by loxp sites. | Clausen BE; Burkhardt C; Reith W; Renkawitz R; Forster I. 1999. Conditional gene targeting in macrophages and granulocytes using LysMcre mice. Transgenic Res 8(4):265-77. | YES(+/+) males with (+/LyC)females. Should never have HOM for LyC. |
|
| 2/7/2017 |
Syn-1 cre IKKB B6N2 | B6.Cg-Tg(Syn1-Cre)671Jxm |
| JAX 003966 | This transgene expresses Cre recombinase under the control of a rat synapsin I promoter. Cre recombinase activity is detected in neuronal cells, including brain, spinal cord and DRGs, as early as E12.5, as well as in neurons in adult. | Zhu Y; Romero MI; Ghosh P; Ye Z; Charnay P; Rushing EJ; Marth JD; Parada LF. 2001. Ablation of NF1 function in neurons induces abnormal development of cerebral cortex and reactive gliosis in the brain. Genes Dev 15(7):859-76.Zhi-Wei Li, Sidne A. Omori, Tord Labuda, Michael Karin and Robert C. Rickert; IKKb Is Required for Peripheral B Cell Survival and Proliferation. J Immunol 2003; 170:4630-4637 | YES(+/+) with (+/TG). Should never have HOM for TG |
|
| 2/7/2017 |
N-Gly alpha 2/ Gly alpha 3 | C57bl/6J-Glycine R a2/a3 KO | 1.10/2016 | Homanics from U Pittsburg |
| No papers published. | Various breeding schemes used in an attempt to obtain WHAT??. Glya2 is on X chromosome. |
|
| 4/20/2017 |
SCN4B KO B6N2 |
| 1/24/2014 | Homanics | Scn4b KO. Exon 2 of Scn4b gene is deleted via Cre recombination. 5' loxp site still remaining. | No papers published. | YES - Het breeding regular: 25%,50%,25% |
|
| 11/30/2017 |
Gabba RHO1 KI |
| 4/8/2015 | Homanics | The knockin (KI) allele harbors 3 bp changes that alter 1 amino acid (T298Y) of the GABAA-R rhoI subunit gene. The KI was created using CRISPR/Cas9 on a pure C57BL/6J (JAX) background. The line was derived from founder #4109. | No papers published. | YES - Het breeding regular: 25%,50%,25% |
|
| 12/21/2017 |
GABRR2 KO B6N3 | Gabrr2tma1a(KOMP)Wtsi | 6/10/2013 | KOMP |
| No papers published. | YES- Het breeding |
|
| 1/18/2018 |
Gabba Rho1 | B6;129S4-Gabrr1tm1Llu/J | 7/12/2009 | JAX 010535 | Gaba rho1 KO. Targeted deletion of exon 1 (and surrounding sequence) of GABA rho1 gene. Neo cassette remains. | Zheng W; Xie W; Zhang J; Strong JA; Wang L; Yu L; Xu M; Lu L. 2003. Function of gamma-aminobutyric acid receptor/channel rho 1 subunits in spinal cord. J Biol Chem 278(48):48321-9. | YES - Het breeding regular: 25%,50%,25% |
|
| 1/18/2018 |
Gly alpha 3 B6N2 | Glycine Alpha 3 KO | 11/15/2011 | Germany (Bodo Laube) | Glra3 KO. Targeted deletion of exon 7 of the glycine receptor alpha 3 subunit. Neo cassette has been removed. | Harvey RJ, Depner UB, Wässle H, Ahmadi S, Heindl C, Reinold H, Smart TG, Harvey K, Schütz B, Abo-Salem OM, Zimmer A, Poisbeau P, Welzl H, Wolfer DP, Betz H, Zeilhofer HU, Müller U. GlyR alpha3: an essential target for spinal PGE2-mediated inflammatory pain sensitization. Science. 2004 May 7;304(5672):884-7. | YES - Het breeding regular: 25%,50%,25% |
|
| 1/24/2018 |
Lyz2-Cre | B6.129P2-Lyz2tm1(cre)Ifo/J |
| JAX 004781 | The LysMcre knock-in/knockout allele has a nuclear-localized Cre recombinase inserted into the first coding ATG of the lysozyme 2 gene (Lyz2); both abolishing endogenous Lyz2 gene function and placing NLS-Cre expression under the control of the endogenous Lyz2 promoter/enhancer elements. | Clausen BE; Burkhardt C; Reith W; Renkawitz R; Forster I. 1999. Conditional gene targeting in macrophages and granulocytes using LysMcre mice. Transgenic Res 8(4):265-77. |
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Gly alpha 2 B6N2 | Glyra2 | 8/14/2009 | University of Louisville | Glra2 KO. Targeted deletion of exons 6 and 7 of the glycine alpha 2 receptor. Neo cassette remains. | Young-Pearse TL, Ivic L, Kriegstein AR, Cepko CL. Characterization of mice with targeted deletion of glycine receptor alpha 2. Mol Cell Biol. 2006 Aug;26(15):5728-34. | YES -Males(+/+) with Female (+/-). Gene is on X chromosome. Should never have Het males or KO females |
|
| 1/8/2019 |
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| 7/5/2017 | Homanics from U Pittsburg | TX3 = mouse lncPpara = human Linc00899. Mouse was made with CRISPOR. Approximately 1.3kb has been deleted from the TX3 locus, including the promoter and all of exon 1. See "TX3 mouse making.docx" for detailed info. |
| YES- Het breeding | TX3 KO |
| 3/1/2019 |
Sun1-GFP-Camk2a-Cre |
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| Presence of Cre will remove a loxP transcriptional roadblock and allow expression of Sun1-GFP in excitatory neurons |
| Cross of our Sun1-GFP and Camk2a-Cre colonies |
|
| 3/13/2019 |
Glyra2 KI B6N2 | C57bl/6J--Glyr2KI | 4/18/2014 | Homanics from U Pittsburg | Mouse should be designated Glra2 KI [L298A]. Mouse was created using TALENs. KI contains a Leu [CTT] -> Ala [GCG] mutation at codon 298 plus 2 additional silent mutations in codons 291 [GAC -> GAT] and 295 [GCG -> GCT]. The silent mutation at codon 291 creates an Eco RV site not present in the WT sequence. See "L298A mouse production details.docx" for more info. | No papers published. | YES -Males(+/+) with Female (+/-). Gene is on X chromosome. Should never have Het males or KO females | Glra2 KI |
| 6/19/2019 |
Gt(ROSA)26Sor-Sun1-GFP-Myc | B6;129-Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat/J | 1/9/2017 | JAX 021039 | These Sun1-tagged mice are used in the INTACT (isolation of nuclei tagged in specific cell types) method for immunopurification of nuclei. These mice contain a CAG promoter driving expression of coding sequences for the mouse nuclear membrane protein SUN1 (Sad1 and UNC84 domain containing 1) fused at its C-terminus to 2 copies of superfolder GFP (sfGFP) followed by 6 copies of Myc, inserted in the Gt(ROSA)26Sor locus. Expression of Sun1-sfGFP-Myc is prevented by an upstream loxP-flanked stop cassette. Cre-dependent removal of the loxP-STOP-loxP cassette allows expression of the SUN1 fusion protein at the inner nuclear membrane in targeted cell types. These nuclei can be immunopurified with antibodies against GFP or MYC for transcriptional and epigenetic studies. | Mo A, Mukamel E2, Davis FP, Luo C, Henry GL, Picard 3, Urich MA, Nery J4, Sejnowski TJ, Lister 6, Eddy SR, Ecker JR, Nathans J.Epigenomic Signatures of Neuronal Diversity in the Mammalian Brain.Neuron. 2015 Jun 17;86(6):1369-84. | Homozygous breeding | Sun1-GFP |
| 7/5/2019 |
Camk2a-Cre | B6.Cg-Tg(Camk2a-cre)T29-1Stl/J | 1/9/2017 | JAX 005359 | These transgenic mice express the Cre recombinase under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha promoter. Cre recombinase expression is detected in the forebrain, specifically to the CA1 pyramidal cell layer in the hippocampus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination occurs in the pyramidal cell layer. | Tsien JZ; Chen DF; Gerber D; Tom C; Mercer EH; Anderson DJ; Mayford M; Kandel ER; Tonegawa S. 1996. Subregion- and cell type-restricted gene knockout in mouse brain [see comments] Cell 87(7):1317-26. | Homozygous breeding | Camk2a-Cre |
| 7/5/2019 |
TRIF-CK0-TM8 | C57Bl/6J- TRIF-CKO-TM8 |
| Homanics/UT-MGEF |
|
| YES- Het breeding | Trif--floxed or Trif-cKO, depending on Cre status |
| All cages transferred to ARC 2.130 May 2019 |
TRIF-CKO-TM1 | C57Bl/6J- TRIF-CKO-TM1 |
| Homanics/UT-MGEF |
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| YES- Het breeding |
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| All cages transferred to ARC 2.128 July 2019 |
MYD88-GLOBAL-KO | B6.129P2(SJL)-Myd88^tm1.1Defr/J | 9/8/2014 | JAX 009088 | The Myd88-deficient allele encodes a deletion of exon 3 of the myeloid differentiation primary response gene 88 locus. | Hou B; Reizis B; Defranco AL. 2008. Toll-like Receptors Activate Innate and Adaptive Immunity by using Dendritic Cell-Intrinsic and -Extrinsic Mechanisms. Immunity 29(2):272-82 | Homozygous breeding |
|
| 8/9/2019 |