Mayfield_MBB_LSSE_Fall_2023

Mayfield_MBB_LSSE_Fall_2023

Fall 2023 Lab Safety Self Evaluation

 

Principal Investigator's (PI) First Name

R. Dayne

Principal Investigator's (PI) Last Name

Mayfield

PI's EID

rdmayfie

PI's Email

dayne.mayfield@austin.utexas.edu

Building

MBB, MOFFETT MOLECULAR BIOLOGY BLDG.

Lab Room Numbers(s)

MBB 1.106, MBB 1.106A, MBB 1.118, MBB 1.122, MBB 1.124, MBB 1.124B, MBB 1.132, MBB 1.136, MBB 1.150

College

College of Natural Sciences

Department

Waggoner Ctr. for Alcohol and Addiction

Submitter (if different from PI)

Jessica McFarland

Does your lab care about Green Labs/Lab Sustainability?

Yes

Is your lab group interested in becoming a certified Green Lab?

Not Sure

Are any personnel working in the lab unpaid (volunteers)?

Yes

Does your lab have cryogens (e.g., liquid nitrogen, liquid helium)?

Yes, <100L (small dewar)

Does your lab have cryogenic containers with pressure gauges that you refill on campus?

No

Does your lab have nanoparticles?

No

Does your lab have any transgenic animals?

No

Does your lab have any transgenic plants?

No

All lab incidents, accidents, and injuries are reported to EHS and your PI and/or supervisor.

Yes

All lab personnel are aware of the new online Incident Notification system.

Yes

Lab personnel have conducted risk assessments for experiments.

Yes

Lab personnel have developed written SOPs for chemicals/procedures.

Yes

Safety is discussed at all lab meetings.

Yes

Lessons learned are reviewed in the laboratory.

Yes

All lab personnel have reviewed the UT Lab Safety Manual.

Yes

All lab personnel have taken Hazard Communication (OH 101), Laboratory Safety (OH 201), Hazardous Waste (OH 202), Fire Extinguisher (FF 205) training and laboratory Safety Refresher (OH 238, every three years).

Yes

The Site-Specific Hazard Communication training (OH 102) form has been completed and submitted to EHS for all lab personnel.

Yes

During Site-Specific Hazard Communication training (OH 102), all lab personnel are trained to call EHS at 512-471-3511 to report all lab incidents, accidents, and injuries.

Yes

During Site-Specific Hazard Communication training (OH102), all lab personnel are trained on the use and location of the spill kit(s).

Yes

All lab personnel have taken specialized training for hazards in the area where they work (e.g. Bloodborne Pathogens, Basic Radiological Health, X-Ray Safety, Laser Safety, Biological Safety, Dry Ice Shipping, Compressed Gases, Cryogens).

Yes

The emergency contact information on lab signs posted by the entrance is current. Request changes through UT HERD by navigating to the "Placards" icon, selecting the lab, and clicking on the "Edit Lab Placard" button.

Yes

Lab personnel list in UT HERD is current. This information can be found under "Worker Registration" in the "Worker Attached to" table.

Yes

The chemical inventory in UT HERD has been reviewed and updated this semester. You must document your inventory review using the Chemical Inventory Review Statement in the Chemical Inventory box.

Yes

All compressed gases are listed in the chemical inventory in UT HERD.

Yes

The lab-specific biosafety manual has been reviewed within the last year and uploaded into UT HERD. Log in to UT HERD to verify that your uploaded biosafety manual (in SOP Documents) is current and to update the review date.

Yes

Lab locations listed in UT HERD are current. Contact EHS to request changes.

Yes

The UT Austin Lab Attire Policy has been reviewed by lab personnel.

Yes

Lab personnel wear appropriate PPE while in the lab. At a minimum, lab personnel should wear a lab coat, gloves, and eye protection while working in the lab.

Yes

Shorts and open-toed shoes are prohibited in the lab.

Yes

Flame-resistant lab coats are always worn while handling pyrophorics.

N/A

Respirators are present in the lab.

Yes

Lab participates in the Green Labs Kimberly Clark glove recycling program.

No

The hazard information on the lab signs posted by the lab entrance are current. Contact EHS to request changes.

Yes

Food and drink are prohibited in the lab.

Yes

Lab personnel know how to access Safety Data Sheets (SDS).

Yes

Clutter is kept to a minimum in the lab.

Yes

Old or unused lab equipment is sent to Surplus or disposed of.

Yes

Lab walkways are kept free of tripping hazards.

Yes

Access to the lab is controlled when unoccupied (i.e., doors are locked).

Yes

Appropriate containers are used to transport research materials outside the lab.

Yes

Lab personnel are interested in learning how Green Labs can improve lab sustainability.

Yes

Are any chemicals used in the lab?

Yes

All chemicals are stored by hazard class (e.g., flammables, oxidizers, acids, bases, reactives, and toxins) and compatibility.

Yes

Chemical containers are kept closed.

Yes

No more than 10 gallons of flammables are stored outside of a flammable storage cabinet.

Yes

Flammables requiring cold storage are stored in lab-safe refrigerators.

Yes

Refrigerators/Freezers are cleaned out annually and defrosted as needed.

Yes

Acids are stored inside an acid cabinet or plastic secondary containment.

Yes

Peroxide forming chemicals (e.g., THF, ethyl ether, dioxane, cyclohexane, benzyl alcohol) are dated when received and opened.

N/A

Peroxide forming chemicals are disposed of when expired. If no expiration date, these chemicals are disposed of after one year (if unopened) or after 6 months (if opened) of being received.

N/A

Hazardous chemicals are stored at or below eye level. (Consider the height of your lab personnel.)

Yes

Does your lab use ethylene oxide (EtO)?

No

Lab is interested in participating in the Surplus Chemical program.

Yes

Are any compressed gas cylinders used in the lab?

Yes

Toxic gases are stored upright, secured in place, and only in sprinkled, ventilated cabinets or enclosures.

N/A

Lecture bottles and small gas cylinders are stored upright and secured in place.

Yes

Compressed gas cylinders are firmly secured near the middle of the cylinder or near the top and bottom of the cylinder.

Yes

Compressed gas cylinders have safety caps in place when not in use.

Yes

Lab is free of gas tubing running above the ceiling, through doorways, or behind walls.

Yes

Lab personnel are aware that EHS has a tool to remove stuck gas cylinder safety caps.

Yes

Are any biological materials used in the lab?

Yes

Are any animals used in the lab?

No

Open flames are prohibited inside the biosafety cabinet.

Yes

Vacuum lines used with biological materials are protected with an in-line HEPA filter.

Yes

EHS is informed when purchasing, replacing, or moving a biosafety cabinet.

Yes

Biological safety cabinets have been certified within the last year.

Yes

My lab has conducted an inventory of biological agents this year.

Yes

My lab possesses Select Agents or Toxins.

No

The Select Toxin Self-Evaluation form has been completed and submitted to EHS this year. (Required only if your lab possesses Select Agents/Select Toxins.)

N/A

EHS (512-471-3511 or ehs-labstaff@austin.utexas.edu) has been contacted prior to using select agents or toxins in the lab.

N/A

Fabric chairs are prohibited in labs working with biological materials.

Yes

Does your lab utilize an ethylene oxide (EtO) sterilizer?

No

Protocols that have been approved by the appropriate committee are in place for all biological work in the lab.

Yes

List any new biological materials (including pathogens) used in the lab.

N/A

Biosafety Cabinet Information

MBB 1.106A 57787
MBB 1.106A 57838
MBB 1.124 57832

Does your lab have a regulatory permit related to your biological work (e.g., USDA, CDC)?

No

Are any fume hoods present in the lab?

Yes

Fume hoods are working properly. If not working properly, contact the Facilities Service Center at 512-471-2020.

No

Fume hoods only contain materials currently in use (not used for permanent storage).

Yes

Evaporating chemicals (including chemical waste) in the fume hood is prohibited.

Yes

The fume hood sash is closed when not in use and below 18 inches when in use.

Yes

Equipment/materials in the fume hood are elevated 1" or more.

Yes

Fume hood knobs are kept clear (i.e., do not hang lab coats, electrical cords, etc. on fume hood knobs).

Yes

Fume hoods have been certified within the last year (verify sticker on face of fume hood).

Yes

Containers are closed with appropriate lids unless actively adding waste.

Yes

Chemical waste that is stored on the floor is in plastic secondary containment (i.e., not in cardboard or styrofoam).

Yes

Waste containers are in good condition (i.e., no leaks, spills, incompatible material).

Yes

All waste is kept at the point of generation (i.e., waste containers are not moved to another room).

Yes

Lab personnel submit Requests for Disposal (RFDs) regularly so waste does not accumulate in the lab.

Yes

RFDs are submitted through the online waste system EMS

Yes

Each waste container has a properly completed waste tag attached to it at all times (i.e., name, phone number, location, chemical constituents, accumulation start date).

Yes

Only empty, non-hazardous glass is disposed of in the glass waste box (e.g., no liquids, chemicals, paper, gloves, lightbulbs, furnace tubes, etc.).

Yes

Are any sharps used in the lab?

Yes

All sharps (e.g., needles, glass contaminated with biological materials) are deposited into sharps containers.

Yes

Needles are disposed directly into sharps containers. They should not be re-capped, bent, or clipped.

Yes

Sharps containers are closed and submitted to EHS for disposal when ¾ full.

Yes

Chemically-contaminated sharps are segregated from biologically-contaminated sharps.

No

Chemically-contaminated sharps are submitted to EHS as chemical waste.

No

Biologically-contaminated sharps are submitted to EHS as biological waste.

Yes

Glass waste boxes are closed and disposed of through Custodial Services when ¾ full.

Yes

Glass waste boxes are disposed of through Custodial Services when damaged (e.g., water damage, rot, punctures, etc.).

Yes

If the lab recycles gloves, only Kimberly Clark brand nitrile gloves, free of even trace amounts of contamination, are placed in the glove recycling bin.

N/A

All pathological, microbiological, rDNA, and blood/blood product waste is treated in the lab (e.g., autoclaved) or disposed of through EHS.

Yes

If waste is autoclaved, an autoclave record is kept in the EHS log book.

Yes

Blast and face shields are used for high pressure or vacuum experiments.

N/A

All belt driven equipment is protected with belt guards.

N/A

All fans have guards.

N/A

Glassware used below or above atmospheric pressure is taped or shielded.

Yes

Lab equipment is in proper working order and maintained per the manufacturer's instructions.

Yes

Lab prohibits single-pass cooling/processing water.

N/A

Eyewashes are available and unobstructed.

Yes

Eyewashes are flushed weekly and documented monthly.

Yes

Emergency showers and their handles are unobstructed.

Yes

EHS is notified whenever a fire extinguisher is used.

Yes

Spill kits specific to the hazards in your lab are available.

Yes

All lab personnel know the location of the spill kit(s).

Yes

All electrical cords are in good condition (i.e., no cracked/brittle/frayed cords or exposed copper wire).

Yes

Electrical cords are unmodified as provided by the manufacturer (i.e., ground prong not removed, cords not spliced, etc.).

Yes

Use of electrical extension cords in the lab is minimized and temporary (<30 days).

Yes

Lab is free of electrical extension cords and multi-plug power strips running above the ceiling, through doorways, or behind walls.

Yes

Lab is free of unsecured and unprotected electrical extension cords and multi-plug power strips running across the floor where they pose a tripping hazard.

Yes