/
Sample Requirements
Sample Requirements
Sanger Sequencing
A. Individual Samples (<24 samples)
- Use a 1.5 mL microfuge tube
- Provide at least 10 uL of sample (12-15 uL preferred)
- Centrifuge your sample before dropping it off at DSF
- Write your order number and sample NUMBER legibly on the cap
B. 96 Well Plate (>24 samples)
- Use a 96-well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec 951020600)
- Provide at least 10 uL of sample (12-15 uL preferred)
- Seal your plate with adhesive foil (DSF uses VWR 60941-074)
- Centrifuge your plate before dropping it off (you can use our Sorvall Legend X1)
- Write your order number legibly on the plate and foil
- Put your samples in order vertically, starting with well A1
PCR Cleanup
A. Individual Samples (<24 samples)
- Use a 1.5 mL microfuge tube
- Provide at least 20 uL of unpurified PCR reaction
- Centrifuge your sample before dropping it off at DSF
- Write your order number and sample NUMBER legibly on the cap
- Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
- Primer should be provided at 10 uM concentration
- Write the order number AND primer name legibly on the cap.
- Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
B. 96 Well Plate (>24 samples)
- Use a 96-well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec 951020600)
- Provide at least 20 uL of unpurified PCR reaction
- Seal your plate with adhesive foil (DSF uses VWR 60941-074)
- Centrifuge your plate before dropping it off (you can use our Sorvall Legend X1)
- Write your order number legibly on the plate and foil
- Put your samples in order vertically, starting with well A1
- Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
- Primer should be provided at 10 uM concentration
- Write the order number AND primer name legibly on the cap
- Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
Related content
Sanger DNA Sequencing
Sanger DNA Sequencing
More like this
Order Submission
Order Submission
More like this
Template Prep
Template Prep
More like this
Service Types
Service Types
More like this
Results Retrieval
Results Retrieval
More like this
DNA Sequencing Facility
DNA Sequencing Facility
More like this