Immunofluorescent labeling for fresh-frozen, cryosectioned tissue: Adapted by KPA from MK IF protocol for floating sections
Use Immunofluorescence worksheet cryostat.pdfthisImmunofluorescence worksheet cryostat.pdf worksheet.
Set-Up:
EasyDip™ Slide Staining System (EMS) for formaldehyde fixation
- Prepare 4% formaldehyde solution in PBS (see white binder in processing room)
- Using a plethora of dry ice to keep slides cold, locate and confirm existence of all slides to be used in the experiment from -80°C freezer
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