Experimental Protocols

Overview of Harris Lab Methods

Previously used protocols



Notes on "purified water"

Unless otherwise noted, "purified water" mentioned in our protocols refers to the following:

  • ASTM type I, double-distilled, WFI (water for injection), or equivalent. We use this.
    • ASTM type I water can be made with an appropriately maintained water filtration system (e.g., Milli-Q® Type 1 Ultrapure Water System)


Experimental Protocols

Animals

Electrophysiology

Perfusion-fixation

EM Tissue processing

Ultramicrotomy

Electron microscopy - image acquisition

Light Microscopy

Vibratome/Cryostat Sectioning

Others



Protocols under Development (restricted access):