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Small-rna data analysis

Small-rna data analysis

May 03, 2011

  • Running ABI small-rna pipeline on TACC

    • About the pipeline- how it works, advantages and disadvantages.

    • Issues faced - restrictions on data, setting up configuration files, things we tried.

    • Parallelizing the pipeline (way around interactive processes)

    • Results

  • Quantifying mature miRNAs from small-rna pipeline results

    • Our scripts and what they do Normalization ??* Conversion of pipeline output to SAM format ( viewing the reads in base space)

  • Looking for novel miRNA, siRNA, moRNA

    • Generating coverage graphs around each miRNA

    • Could we get Rodney to talk about how they identified that antisense miRNA?