Editing Trace Palette Template

By Kristen Harris (10/14/2010)

Protocol for Editing and Importing a Palette Template to RECONSTRUCT^TM

1.  Create a template that you like in RECONSTRUCT^TM.

   1. Make the top row one measurement and the bottom row another related measurement e.g.:

      1. hd – z-trace of spine head diameter

      2. plen – z trace of spine length

   2.  Save and Transfer to a palette folder and name according to contents, e.g."".

2. Open the Palette Template of interest (e.g."d01 hdplen 1_10.ser") in Windows Notepad

   1. Or other .xml file reader – not WORD or similar word processing program.

3. Under the Edit menu, click Replace... (Ctrl - H)

4. In the Find what entry field, type the old dendrite number (e.g. d01)

5. In the Replace with entry field, type the new dendrite number (e.g. d02 and so on)

6. Click Find Next to verify it is correct

7. Click Replace All

8. Once menu says, Cannot find "d01", click Cancel

9. Under the File menu, click Save As...

10. Type Dxx hdplen 01_10.ser (where Dxx is the new dendrite number) in the File name entry field (e.g. ).

11. To create another set of protrusion numbers, e.g. the hd plen numbers from 11_20 repeat the above, but now you will need to enter the number following the hd or plen for each set from 01 to 11, 02 to 12, 03 to 13, and so on. Save as file name with D01 hdplen 11_20.ser.

    1. Now you can use this as a template for all the rest of the dendrite numbers and repeat itemas 3-10 above now for protrusions 11 through 20 and so on.

12. The SER file is ready to be imported into RECONSTRUCT^TM

    1. For convenience you might want to name your files with the .ser however, D16c cfa 1_10.ser.txt or any name will work as long as the file has the reconstruct format in it.

13. To import into Reconstruct:

    1. Click Series > Import > Series

    2. Uncheck Import Series Settings Option.

    3. Check Import the trace palette

    4. Click Import

Trace Pallette Templates for Download: