Jensen's Media

Mix these ingredients in 800mL of water, pH to 7.3; fill to 950mL and autoclave.

2. Prepare and sterilize these solutions separately (N.B. filter sterilizing seems to yield better growth)

Item	Chemical	Concentration (mM)	Concentration (g/L)	Instructions
1	Glucose	70	12.61	Dissolve 12.61 g in 50mL water
2	MgSO₄· 7H₂O	1.33	0.33	Dissolve 33g in 100mL water
3	CaCl₂ · 2H₂O	0.14	0.021	Dissolve 2.1g in 100mL water
4	FeSO₄ · 7H₂O	0.0039	0.0011	Dissolve 0.11g in 100mL water
5	ZnSO₄ · 7H₂O	0.0085	0.0024	Dissolve 0.24g in 100mL water

3. Add 50 mL glucose and 1 mL each of items 2-5 to the solution prepared in step 1 while stirring.