Jensen's Media
Mix these ingredients in 800mL of water, pH to 7.3; fill to 950mL and autoclave.
Chemical | Concentration (mM) | Concentration (g/L) |
|---|---|---|
NaCl | 85.6 | 5 |
K2HPO4 | 14.4 | 2.51 |
Glutamic acid | 92 | 15.56 |
Valine | 24 | 2.81 |
Phenylalanine | 8 | 1.32 |
2. Prepare and sterilize these solutions separately (N.B. filter sterilizing seems to yield better growth)
Item | Chemical | Concentration (mM) | Concentration (g/L) | Instructions |
|---|---|---|---|---|
1 | Glucose | 70 | 12.61 | Dissolve 12.61 g in 50mL water |
2 | MgSO4 · 7H2O | 1.33 | 0.33 | Dissolve 33g in 100mL water |
3 | CaCl2 · 2H2O | 0.14 | 0.021 | Dissolve 2.1g in 100mL water |
4 | FeSO4 · 7H2O | 0.0039 | 0.0011 | Dissolve 0.11g in 100mL water |
5 | ZnSO4 · 7H2O | 0.0085 | 0.0024 | Dissolve 0.24g in 100mL water |
3. Add 50 mL glucose and 1 mL each of items 2-5 to the solution prepared in step 1 while stirring.