Gel Electrophoresis

Running 1% Agarose DNA Gel

1. Make 50x TAE Buffer

2. Make 1L 1x TAE

3. Put 50mLs 1x TAE in Flask

4. Put 0.5g Agarose in Flask

5. Put paper towel on top of flask to prevent leakage

6. Microwave for 20s, 20 s, and 15s – make sure solutions is homogenous

7. Let set – make sure the gel apparatus and comb are rinsed.

8. Put gel tray in mold and clamp – put comb in gel

9. Add 2.5mL Ethidium Bromide to agarose mix

10. Pour agarose into gel mold and wait for 20 minutes

11. Transfer gel in tray into gel box

12. Pour about 200mLs of 1x TAE buffer (up to the max line) into gel box

13. Add 5mL of Ethidium Bromide to each side of the gel box and mix

14. Prepare DNA samples by mixing with a loading buffer

15. Load DNA samples into the wells with as much space between them as possible

16. Run for 20 to 60 minutes at 100 V

17. Turn off power to stop current

18. Take pictures:

    1. Turn on computer

    2. Click KODAK icon

    3. Click Gel Logic 100GL button

    4. Set gel in light box and close door

    5. Turn on UV light

    6. Click Preview

    7. Adjust aperture and exposure time

    8. Click Capture

    9. Adjust picture as desired – crop, rotate, or color settings

    10. Save and Print